Caspase-10; Human; Recombinant; E. coli

General description

M.W. 11,000 and 18,000 (heterodimer).

Recombinant, human caspase-10 fused at the N- and C- termini to a His Tag™ sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kang, J.J., et al. 1999. J. Biol. Chem. 274, 3189.Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301.Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578.Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.

Packaging

3000 u in Plastic ampoule

Physical form

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, 0.09% APO-10, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-IETD-pNA (catNO=368057">Cat. No. 368057) per min at 25°C, pH 7.4.

Warning

Toxicity: Standard Handling (A)